![]() ![]() In our aim, the present work would help other researchers in the field to reuse the provided datasets for their own scopes, and to combine their creative approaches/analyses to similar acquisitions. Validation of the datasets is conducted by means of established analytical tools to extract the structural and dynamic properties at the sub-cellular scale, such as Single Particle Tracking (SPT) and imaging derived Mean Square Displacement ( iMSD) analyses. early endosomes, late endosomes and lysosomes, along with detailed guidelines to produce analogous experiments. Here we provide exemplary live-cell, time-lapse confocal imaging datasets corresponding to three sub-cellular structures of the endo-lysosomal pathway, i.e. Automated recordings, interactive data acquisition and trigger functions.Īutomatic calculation of specific Raman wavenumbers according to the pumplaser wavelength used for exaction and vice versa.Time-lapse optical microscopy datasets from living cells can potentially afford an enormous amount of quantitative information on the relevant structural and dynamic properties of sub-cellular organelles/structures, provided that both the spatial and temporal dimensions are properly sampled during the experiment. Package to guide you through E-Phys experiments, incl. Wizard for fast and reliable setup of FCS and FLCS experiments and full control of F(L)CS data acquisition. ![]() Wizard for fast and reliable setup of FLIM experiments and full control of FLIM data acquisition. Restriction of detection to a certain time window removes unwanted signal from autofluorescence or backscattering with HyD detectors. Tool to compensate within a z-stack drop of fluorescence intensity that occurs deeper inside your sample with laser power and/or detector gain. Allows Lambda square fluorescence mapping. Imaging method that acquires the full excitation-emission spectrum in each image pixel using a tunable laser and spectral detectors. Imaging method that acquires the emission spectrum in each image pixel using spectral detectors. Includes powerful trigger capabilities such as sending a trigger at a specific channel or position.Ĭonfocal users can select different dyes and LAS X sets up all hardware components accordingly. Incorporate triggers into your time lapse experiments. Send triggers and analog signals to peripherals. Versatile tool set for high content screening and automated microscopy. The step by step wizard guides users through setup, acquisition and quantification of a FRAP experiment Log the environmental data and monitor it during the experiment.Ĭonnect to your acquisition station via web client or mobile device and see the course of your experiment,review the whole experiment or open any image series available in the xperiment treeĪllows system administrators to give different access levels to different LAS X user groups. Have full control of your experimental conditions with the LAS X Environmental Control module. Powerful wizard for FRET acquisition and analysis Histogram based colocalization and area measurements Interactive data recording allowing job-sequencing and online evaluation Module for online ratio measurement, online display of ratio graphs and ratio image Records a movies directly from the live image viewer and stores it in avi format at a user defined location. ![]() Not released for fluorescence applications. Automatically distribute regions and positions to each well/chamber.Īllows the control of any third party camera supported by LAS X.Įffectively sums up only the in focus area of each image in a three dimensional image stack and creates a single EDOF image.ĭetects manual sample movement automatically and smoothly extends the image without the need to click buttons to capture the image. Perform multi well and multi chamber experiments. Includes software autofocus.ĭefine multiple stage locations and revisit them as part of a time-lapse or Z stacking experiment.Ĭreate multiple regions of any shape for overview images and high resolution scans LAS X Core software, operates without the need for a dongle.Īllows the definition of up to 8 acquisition channels per experiment.ĭefine the duration and frequency of image capture for time lapse experiments.įocus positioning and acquisition of 3 dimensional data sets. ![]()
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